| James Doohan | Montgomery (Scotty) Scott |
| Jeffery Hunter | Captain Christopher Pike |
| DeForest Kelley | Dr. Leonard H. McCoy |
| Walter Koenig | Pavel Chekov |
| Mark Lenard | Sarek |
| Nichelle Nichols | Uhura |
| Leonard Nimoy | Spock |
| William Shatner | Captain James T. Kirk |
| George Takei | Hikaru Sulu |
| Grace Lee Whitney | Janice Rand |
| Brian Bonsall | Alexander |
| LeVar Burton | Geordi LaForge |
| Denise Crosby | Tasha Yar and Sela |
| Jonathan Frakes | Commander William T. Riker |
| Michelle Forbes | Ro Laren |
| Whoopi Goldberg | Guinan |
| Gates McFadden | Doctor Beverly Crusher |
| Diana Muldaur | Doctor Katherine Pulaski |
| Dwight Schultz | Reginald Barclay |
| Marina Sirtis | Counselor Deanna Troi |
| Brent Spiner | Data |
| Patrick Stewart | Captain Jean-Luc Picard |
| Wil Wheaton | Wesley Crusher |
| Patti Yasutake | Nurse Alyssa Ogawa |
| Cecily Adams | Ishka |
| Marc Alaimo | Gul Dukat |
| Rene Auberjoinois | Odo |
| Felecia Bell | Jennifer Sisko |
| Casey Biggs | Damar |
| Avery Brooks | Captain Benjamin Sisko |
| Rosalind Chao | Keiko O'Brien |
| Jeffery Combs | Weyoun and Brunt (Shran- Enterprise) |
| Nicole de Boer | Ezri Dax |
| James Darren | Vic Fontaine |
| Michael Dorn | Worf |
| Terry Farrell | Jadzia Dax |
| Louise Fletcher | Kai Winn |
| Max Grodénchik | Rom |
| J. G. Hertzler | Martok |
| Barry Jenner | Admiral Ross |
| Salome Jens | female Founder |
| Penny Johnson | Kasidy Yates |
| Cirroc Lofton | Jake Sisko |
| Chase Masterson | Leeta |
| Colm Meaney | Chief Miles O'Brien |
| Robert O'Reilly | Gowron |
| F. J. Rio | Muniz |
| Andrew Robinson | Garak |
| Camille Saviola | Kai Opaka |
| Armin Shimerman | Quark |
| Alexander Siddig | Dr. Julian Bashir |
| Nana Visitor | Colonel Kira Nerys |
| Robert Beltran | Chakotay |
| Roxann Dawson | B'Elanna Dawson |
| Brad Dourif | Lon Suder |
| Alexander Enberg | Ensign Vorik |
| Martha Hackett | Seska |
| Jennifer Lien | Kes |
| Robert Duncan McNeill | Tom Paris |
| Kate Mulgrew | Captain Kathryn Janeway |
| Ethan Phillips | Neelix |
| Robert Picardo | The Doctor |
| Scarlett Pomers | Naomi Wildman |
| Jeri Ryan | Seven of Nine |
| Tim Russ | Tuvok |
| Garrett Wang | Harry Kim |
| Vaughn Armstrong | Admiral Forrest and other roles |
| Scott Bakula | Captain Jonathan Archer |
| John Billingsley | Doctor Phlox |
| Jolene Blalock | T'Pol |
| Dominic Keating | Malcom Reed |
| Anthony Montgomery | Travis Mayweather |
| Linda Park | Hoshi Sato |
| Connor Trinneer | Charles "Trip" Tucker III |
| F. Murray Abraham | Ad'har Ru'Afo (Insurrection) |
| Kirstie Alley | Lieutenant Saavik (Wrath of Khan) |
| Bibi Besch | Doctor Carol Marcus (Wrath of Khan) |
| Merritt Butrick | Dr. David Marcus (Wrath of Khan and Search for Spock) |
| James Cromwell | Zefram Cochrane (First Contact) |
| Robin Curtis | Lieutenant Saavik (Search for Spock and Voyage Home) |
| Daniel Hugh Kelly | Sojef (Insurrection) |
| Persis Khambatta | Lieutenant Ilia (Motion Picture) |
| Alice Krige | Borg Queen (First Contact) |
| Laurence Luckinbill | Sybok (Final Frontier) |
| Neal McDonough | Lieutenant Hawk (First Contact) |
| Malcolm McDowell | Doctor Tolian Solan (Generations) |
| Barbara March | Lursa (Generations) |
| Ricardo Montalban | Khan (The Wrath of Khan) |
| Donna Murphy | Anji (Insurrection) |
| Alan Ruck | Captain John Harriman (Generations) |
| Gwynyth Walsh | B'Etor (Generations) |
| Michael Welch | Artim (Insurrection) |
| Alfre Woodard | Lily Sloane (First Contact) |
| Jane Wyatt | Amanda (Voyage Home) |
| Anthony Zerbe | Adm. Mathew Dougherty (Insurrection) |
Other
| John de Lancie | Q |
| Rick Worthy | Multiple Roles |
| Ira Steven Behr | Producer and Writer |
| Rick Berman | Producer and Co-Creator |
| Brannon Braga | Executive Producer |
| Rene Echevarria | Producer and Writer |
| Ronald D. Moore | Producer and Writer |
| Denise Okuda | Scenic Artist and Video Supervisor |
| Michael Okuda | Scenic Art Supervisor |
| Michael Piller | Co-Creator, Producer, and Writer |
| Gene Roddenberry | Creator |
| Majel Roddenberry | Nurse Christine Chapel, Lwaxana Troi |
| Rick Sternbach | Senior Illustrator |
| Jeri Taylor | Co-Creator, Producer, and Writer |
| Herman Zimmerman | Production Designer |